Modified salting‐out method for DNA isolation from newborn cord blood nucleated cells
نویسندگان
چکیده
منابع مشابه
Nucleated red blood cells in the fetus and newborn.
Although nucleated red blood cells (nRBCs) are rarely found circulating in older children, they are commonly seen in the blood of newborns. They are primarily produced in the fetal bone marrow in response to erythropoietin and are stored in the marrow as precursors to reticulocytes and mature erythrocytes. Many acute and chronic stimuli cause increases in the number of circulating nRBCs from ei...
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Placental/umbilical cord blood (PCB) is a source of hematopoietic stem cells for bone marrow reconstitution. Engraftment speed and survival are related to the total nucleated cell (TNC) dose of the graft. This study explored the possible influence on engraftment of nucleated red blood cells (NRBCs) in the graft. Automated hematology analyzers were used to enumerate TNCs. NRBCs were counted by v...
متن کاملIsolation of multipotent mesenchymal stem cells from umbilical cord blood.
It is well accepted that umbilical cord blood has been a source for hematopoietic stem cells. However, controversy exists as to whether cord blood can serve as a source of mesenchymal stem cells, which can differentiate into cells of different connective tissue lineages such as bone, cartilage, and fat, and little success has been reported in the literature about the isolation of such cells fro...
متن کاملIsolation of mesenchymal stem cells from equine umbilical cord blood
BACKGROUND There are no published studies on stem cells from equine cord blood although commercial storage of equine cord blood for future autologous stem cell transplantations is available. Mesenchymal stem cells (MSC) have been isolated from fresh umbilical cord blood of humans collected non-invasively at the time of birth and from sheep cord blood collected invasively by a surgical intrauter...
متن کاملEvaluation of different approaches for fetal DNA analysis from maternal plasma and nucleated blood cells.
Analysis from Maternal Plasma and Nucleated Blood Cells, Maddalena Smid, Fiorenza Lagona, Lara de Benassuti, Augusto Ferrari, Maurizio Ferrari, and Laura Cremonesi (Istituto di Ricovero e Cura a Carattere Scientifico, Hospital San Raffaele, 1 Department of Obstetrics and Gynecology and 2 Unit of Genetics and Molecular Diagnostics, Via Olgettina 60, 20132 Milan, Italy; * address correspondence t...
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ژورنال
عنوان ژورنال: Journal of Clinical Laboratory Analysis
سال: 2000
ISSN: 0887-8013,1098-2825
DOI: 10.1002/1098-2825(20001212)14:6<280::aid-jcla6>3.3.co;2-s